Methods and products for transfecting cells
AssigneeFactor Bioscience Inc.
InventorsMatthew Angel, Christopher Rohde
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Abstract
The present invention relates in part to nucleic acids encoding proteins, nucleic acids containing non-canonical nucleotides, therapeutics comprising nucleic acids, methods, kits, and devices for inducing cells to express proteins, methods, kits, and devices for transfecting, gene editing, and reprogramming cells, and cells, organisms, and therapeutics produced using these methods, kits, and devices. Methods for inducing cells to express proteins and for reprogramming and gene-editing cells using RNA are disclosed. Methods for producing cells from patient samples, cells produced using these methods, and therapeutics comprising cells produced using these methods are also disclosed.
Claims19
A method for preparing a gene-edited hematopoietic cell, the method comprising transfecting a hematopoietic cell with an in vitro transcribed synthetic RNA molecule encoding a gene-editing protein, wherein the hematopoietic cell expresses the gene-editing protein, and wherein the gene-editing protein comprises a DNA-binding domain and a nuclease catalytic domain that causes a double-strand break in the DNA of the hematopoietic cell to reduce the function of a CCR5 gene, a CXCR4 gene, or both.
Dependencies are inferred from the claim text.