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AssigneeIowa State University Research Foundation, Inc.
InventorsLing Li, Eve Syrkin Wurtele
Estimating the term…
Method of increasing protein content in a eukaryotic cell comprising an NF-YC4 gene comprising modifying the transcriptional repressor binding site; method of producing a plant with increased protein content comprising crossing and selecting for increased protein content; method of increasing resistance to a pathogen or a pest in a plant cell or plant comprising an NF-YC4 gene comprising modifying the transcriptional repressor binding site, alone or in further combination with expressing QQS in the plant cell or plant; method for producing a plant with increased resistance to a pathogen or a pest comprising crossing and selecting for increased resistance to the pathogen or the pest; a cell, collection of cells, tissue, organ, or organism, such as a plant, in which the NF-YC4 gene comprises a promoter comprising a transcriptional repressor binding site that has been modified so that the transcriptional repressor cannot prevent transcription of the NF-YC4; plants and hybrids thereof; and seeds.
A genetically modified plant, or a part from the genetically modified plant, comprising a genetically modified endogenous nuclear factor Y subunit C4 (NF-YC4) promoter operably linked to an endogenous NF-YC4 protein coding sequence, wherein an agent for modifying a nucleotide sequence of said NF-YC4 promoter, modifies at least one transcriptional repressor binding site in the nucleotide sequence of said NF-YC4 promoter, and whereupon the transcriptional repressor cannot prevent transcription of the NF-YC4 coding sequence and thus the modified endogenous NF-YC4 promoter increases the transcription of the endogenous NF-YC4 protein coding sequence in the genetically modified plant or the part from the genetically modified plant, and wherein said genetically modified plant or said part from the genetically modified plant that was modified to increase NF-YC4 expression was selected for increased total protein content due to overexpression of said endogenous NF-YC4 protein as compared to the total protein content in the corresponding wild-type (WT) plant or the corresponding WT part thereof of the same plant species lacking said modified NF-YC4 promoter, and wherein said genetically modified plant, or said part from the genetically modified plant, is non-naturally occurring.
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