FAD2 genes and mutations
AssigneesCibus US LLC, Cibus Europe B.V.
InventorsGregory F. W. Gocal, Mark Knuth
Life of this patent
Estimating the term…
Abstract
The present disclosure provides fatty acid desaturase 2 (FAD2) genes and plants and/or plant cells bearing one or more mutations in two or more FAD2 genes; as well as methods of making and using such plants. In some embodiments, plants producing seed oil with high oleic acid content are provided.
Claims14
A Brassica napus plant or part thereof comprising a homozygous loss-of-function mutation in three endogenous genes encoding fatty acid desaturase 2 (FAD2) polypeptides having the coding sequences of SEQ ID NO:
- 1, SEQ ID NO:
- 2, and SEQ ID NO:
- 3, and wherein the plant or part thereof comprises a FAD2 gene having the coding sequence of SEQ ID NO:
- 4 which encodes a functional FAD2 polypeptide, and wherein seed oil produced from the plant exhibits the following characteristics:
- 1) contains oleic acid at a level of at least 80% by weight of the total fatty acid content of the seeds;
- 2) contains linoleic acid at a level less than 4% by weight of the total fatty acid content of the seeds;
- 3) contains linolenic acid at a level less than 5% by weight of the total fatty acid content of the seeds;
- 4) the ratio of the quantity of linoleic acid to linolenic acid is less than 1;
- and
- 5) has increased oxidative stability for at least 32 hours as compared to a corresponding control.
Oil extracted from seeds of Brassica napus plants comprising a homozygous loss-of-function mutation in three endogenous genes encoding fatty acid desaturase 2 (FAD2) polypeptides having the coding sequences of SEQ ID NO:
- 1, SEQ ID NO:
- 2, and SEQ ID NO:
- 3, and wherein the Brassica napus plants comprise a FAD2 gene having the coding sequence of SEQ ID NO:
- 4 which encodes a functional FAD2 polypeptide, and wherein the oil exhibits the following characteristics:
- 1) contains oleic acid at a level of at least 80% by weight of the total fatty acid content of the seeds;
- 2) contains linoleic acid at a level less than 4% by weight of the total fatty acid content of the seeds;
- 3) contains linolenic acid at a level less than 5% by weight of the total fatty acid content of the seeds;
- 4) a ratio of the quantity of linoleic acid to linolenic acid less than 1;
- and
- 5) has increased oxidative stability for at least 32 hours as compared to a corresponding control.
A method for producing seed oil in a seed, said method comprising growing a Brassica napus plant comprising a homozygous loss-of-function mutation in at least three endogenous genes encoding FAD2 polypeptides, wherein the endogenous genes comprise a coding sequence selected from the group consisting of having the coding sequences of SEQ ID NO:
- 1, SEQ ID NO:
- 2, and SEQ ID NO:
- 3, and wherein the plant comprises a FAD2 gene having the coding sequence of SEQ ID NO:
- 4 which encodes a functional FAD2 polypeptide, and wherein said Brassica napus plant produces seeds, and wherein seed oil produced from the plant exhibits the following characteristics:
- 1) contains oleic acid at a level of at least 80% by weight of the total fatty acid content of the seeds;
- 2) contains linoleic acid at a level less than 4% by weight of the total fatty acid content of the seeds;
- 3) contains linolenic acid at a level less than 5% by weight of the total fatty acid content of the seeds;
- 4) the ratio of the quantity of linoleic acid to linolenic acid is less than 1;
- and
- 5) has increased oxidative stability for at least 32 hours as compared to a corresponding control.
Dependencies are inferred from the claim text.