Methods for producing transformed plants
AssigneeFraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V.
InventorsSruthy Maria Augustine, Anoop Vadakan Cherian, Kerstin Seiling, Nicole Raven, Stefano Di Fiore, Stefan Schillberg, Ulrich Commandeur
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Abstract
Methods for highly efficient and fast generation of transformed plants, highly efficient, mild and fast generation methods for introducing a biomolecule into an intact plant cell by a laser-assisted transfection method, and the regeneration of cell lines, whole tissues or organisms thereof.
Claims17
A method of producing a transformed plant by introducing an expression vector into an intact plant cell with a laser-assisted transfection method comprising the steps of:
- (i) providing an intact plant or a plant explant isolated from any organ and or tissue of a plant and comprising intact plant cells or an isolated plant cell with a cell wall;
- (ii) providing an expression vector and a selectable marker;
- (iii) delivering said expression vector and the selectable marker into the plant cell by a laser-assisted transfection method, wherein the cell or tissue is surrounded by a liquid, and the laser focal point is within the liquid adjacent to the cell with a cell wall and not directly on the cell with a cell wall;
- (iv) identifying the cells transfected with the expression vector;
- and
- (v) selecting the cells transfected with the expression vector and regenerating the cells to intact plantlets by growing in and/or on a growth media.
A method for introducing a biomolecule into an intact plant cell by a laser-assisted transfection method comprising the steps of:
- (i) providing a plant explant isolated from any organ and or tissue of a plant and comprising intact plant cells or an isolated plant cell with a cell wall and a selectable marker;
- (ii) providing a biomolecule and a selectable marker;
- and
- (iii) delivering said biomolecule and the selectable marker into the plant cell by a laser-assisted transfection method, wherein the cell or tissue is surrounded by a liquid and the laser focal point is within the liquid adjacent to the cell with a cell wall and not directly on the cell with a cell wall.
Dependencies are inferred from the claim text.