RNase inhibitors
AssigneeNew England Biolabs, Inc.
InventorsNathan Tanner, Jennifer Ong, Esta Slayton, Lisa L. Maduzia, Salvatore V. Russello
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Abstract
Compositions, methods and kits are provided that include an inhibitory oligonucleotide RNase inhibitor capable of inhibiting one or more types of RNase that coexist with biological samples or are introduced in the laboratory, thereby protecting RNA in the sample from degradation. More than one type of oligonucleotide RNase inhibitor may be combined in a mixture to inhibit a plurality of different RNases. Single oligonucleotides were identified to have inhibitory activity for a plurality of different RNases. The RNase oligonucleotide inhibitor may be immobilized on beads or other surface. It may be stored in a lyophilized form or in solution.
Claims13
A composition, comprising:
- a mixture comprising two or more single stranded oligonucleotide RNase inhibitors, wherein each RNAse inhibitor inhibits the activity of at least one of RNase A and RNase I, wherein the RNase inhibitors are selected from the group consisting of:
- (a) an oligonucleotide having at least 90% sequence identity with a sequence selected from any of SEQ ID NOs:
- 1-62;
- and
- (b) an oligonucleotide having at least 90% sequence identity with a sequence selected from any of SEQ ID NOs:
- 1-62, further comprising one or more hydrophobic nucleotide substitutions in the oligonucleotide.
Dependencies are inferred from the claim text.