Methods and compositions for linking RNA stem loops
AssigneeThe Regents of the University of California
InventorsNeal Devaraj, Dongyang Zhang
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Abstract
Provided herein are, inter alia, methods and compositions for linking RNA stem loops. The methods include linking a first RNA stem loop and a second RNA stem loop by way of a preQ1 linking compound.
Claims20
A method of linking a first RNA stem loop oligonucleotide and a second RNA stem loop oligonucleotide, the method comprising contacting the first RNA stem loop oligonucleotide and the second RNA stem loop oligonucleotide with a PreQ1 linking compound and a tRNA-guanine transglycosylase (TGT) enzyme,
- wherein the PreQ1 linking compound has the formula:
- wherein L1 is a covalent linker;
- wherein said first RNA stem loop oligonucleotide comprises a first guanosine in a first RNA loop portion of a first RNA stem loop;
- and
- wherein said second RNA stem loop oligonucleotide comprises a second guanosine in a second RNA loop portion of a second RNA stem loop.
A method of linking a first RNA stem loop of an RNA stem loop oligonucleotide and a second RNA stem loop of said RNA stem loop oligonucleotide, the method comprising contacting said RNA stem loop oligonucleotide with a PreQ1 linking compound and a tRNA-guanine transglycosylase (TGT) enzyme,
- wherein said PreQ1 linking compound has the formula:
- wherein L1 is a covalent linker;
- and
- wherein said first RNA stem loop comprises a first guanosine in a first RNA loop portion of the first RNA stem loop, and
- wherein said second RNA stem loop comprises a second guanosine in a second RNA loop portion of the second RNA stem loop.
An RNA compound having the formula:
- wherein
- L1 is a covalent linker;
- R1 is a first RNA loop portion of a first RNA stem loop;
- and
- R2 is a second RNA loop portion of a second RNA stem loop.
Dependencies are inferred from the claim text.