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US 12,559,730 B2Granted
Mutant KLF protein, and method for producing induced pluripotent stem cells
AssigneesRiken, University of Tsukuba
InventorsYohei Hayashi, Evgeniia Borisova, Koji Hisatake, Ken Nishimura, Fumiaki Yumoto
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Abstract
There is provided a mutant KLF protein that can induce reprogramming of a somatic cell at a higher efficiency than a KLF protein having a natural amino acid sequence. There is also provided a method for efficiently producing an iPS cell by using the mutant KLF protein. There is provided a mutant KLF protein having an amino acid substitution, or a peptide fragment thereof containing the amino acid substitution.
Claims19
A mutant Krüppel-like factor (KLF) protein comprising an amino acid substitution, wherein
- the amino acid substitution is a substitution of any of the following:
- (a) serine at position 349 and/or leucine at position 356 in the amino acid sequence set forth in SEQ ID NO:
- 1;
- (b) serine at position 342 and/or leucine at position 349 in the amino acid sequence set forth in SEQ ID NO:
- 3;
- (c) serine at position 500 and/or leucine at position 507 in the amino acid sequence set forth in SEQ ID NO:
- 5;
- or
- (d) serine at position 443 and/or leucine at position 450 in the amino acid sequence represented by SEQ ID NO:
- 7.
7 more elements
Dependencies are inferred from the claim text.