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US 12,559,722 B2Granted
Human disc tissue
AssigneeDiscGenics, Inc.
InventorsValery Kukekov, Umar Akbar, Christopher D. Duntsch
Life of this patent
Estimating the term…
Abstract
This invention provides disc stem cells, processes for obtaining and culturing disc stem cells, and methods for repairing damaged or diseased disc tissue comprising the use of the disc stem cells of the invention.
Claims28
A method of treating a subject suffering from a degenerative condition of a specific tissue with a tissue specific stem cell, comprising the steps of:
- a. suspending a sphere-like cluster of stem cells or an isolated stem cell from said sphere-like cluster in a medium comprising 6-10% serum, basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) and lacking methylcellulose, wherein the cluster of stem cells or isolated stem cell are derived from annulus pulposus, nucleus pulposus, transition zone or articular cartilage;
- b. distributing the suspension onto ultra-low binding culture plates;
- c. culturing said suspension for 8-16 days to produce a mature sphere of heterogeneous morphology having a diameter of roughly 100-600 microns, wherein the mature sphere comprises at least one tissue-specific stem cell selected from the group consisting of annulus pulposus, nucleus pulposus, transition zone, or articular cartilage;
- and
- d. isolating the at least one tissue-specific stem cell;
- and
- e. administering the at least one tissue-specific stem cell to said subject at or near articular cartilage, a joint, or an intervertebral disc, thereby treating the subject's degenerative condition.
4 more elements
A method of treating a subject suffering from a degenerative condition of a specific tissue with a tissue specific stem cell, comprising the steps of:
- a. suspending a sphere-like cluster of stem cells or an isolated stem cell from said sphere-like cluster in a medium comprising 6-10% serum, basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) and lacking methylcellulose, wherein the cluster of stem cells or isolated stem cell are derived from annulus pulposus, nucleus pulposus, transition zone, or;
- b. distributing the suspension onto ultra-low binding culture plates;
- c. culturing said suspension for 8-16 days to produce a mature sphere of heterogeneous morphology containing approximately 100-500 cells, wherein the mature sphere comprises at least one tissue specific stem cell of annulus pulposus, nucleus pulposus, transition zone, or;
- and
- d. isolating the at least one tissue specific stem cell;
- and
- e. administering the at least one stem cell to said subject at or near articular cartilage, a joint, or an intervertebral disc, thereby treating the subject's degenerative condition.
4 more elements
Dependencies are inferred from the claim text.