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US 12,559,713 B2Granted
Fermentation process
AssigneeGlaxosmithkline Biologicals SA
InventorsPhilippe Dehottay, Romain Kocks, Quentin Zune
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Abstract
The present invention relates to processes for Bordetella fermentation and virulence factor production, especially PT production, for large scale manufacturing. More particularly, processes including a medium conditioning step carried out prior to inoculation.
Claims15
A process for producing a conditioned growth medium for the cultivation of Bordetella pertussis species and/or for the production of Bordetella pertussis proteins comprising:
- a. providing a sterile growth medium:
- b. holding the sterile growth medium at a temperature between about 28 and about 35° C. for about 20 to 35 hours;
- and
- c. stirring and/or aerating the sterile growth medium to produce an oxygen volumetric mass transfer coefficient (kLa) of about 10 h−1 to about 130 h−1, thereby providing the conditioned growth medium for the cultivation of Bordetella pertussis species and/or for the production of Bordetella pertussis proteins, wherein the growth medium is modified Stainer-Scholte (MSS).
1 more element
Dependencies are inferred from the claim text.