Method for extracting mupirocin

A method for extracting mupirocin comprising:

• a. resin adsorption:
• adding the resin into a mupirocin fermentation broth and stirring for adsorption, filtering the fermentation broth, rinsing, and collecting the resin, wherein the resin is a nonpolar macroporous adsorption resin, the feeding amount of the resin is 0.02 to 0.05 g mupirocin/g resin, and the pH of the resin adsorption is 5.5 to 6.5;
• b. desorption and concentration:
• loading the resin collected in step a on a desorption column;
• soaking the resin with ethyl acetate or acetone, desorbing, and collecting and concentrating the desorption solution;
• and
• c. extraction:
• extracting the concentrated desorption solution in step b, and separating a solvent phase;
• wherein the extraction is a multi-step extraction comprising a first ester solvent extraction, at least one alkaline water extraction, and a second ester solvent extraction in order;
• wherein the first and second ester solvent is ethyl acetate, and the pH of the first and second ester solvent extractions is 3.0 to 5.0;
• the alkaline water is selected from a sodium bicarbonate solution, an ammonium bicarbonate solution or a sodium hydroxide solution, and the pH of the alkaline water is 7.5 to 10.0;
• wherein the alkaline water extraction is repeated once or twice or more times, and the alkaline water phases separated from the extractions are combined for use in the second ester solvent extraction.