Method of delivering proteins into cells
AssigneeGuangzhou Lide Biomedicine Technology Co., Ltd.
InventorsZhijia Liu, Zhicheng Le, Yongming Chen
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Abstract
The present application discloses a method of delivering a protein drug into a cell, including utilization of a cationic lipid analog material. The cationic lipid analog material of the present application has an efficient intracellular delivery of a protein, and is effective for proteins of different molecular weights and charges. Moreover, the biological activity of the protein can still be maintained when the protein is delivered into the cell. At the same time, the ionizable cationic lipid analog material has low toxicity to cells and good biocompatibility, and can be used as delivery carriers for protein drugs.
Claims18
A method of delivering a protein drug into a cell, comprising utilization of a cationic lipid analog material, wherein the cationic lipid analog material is an ionizable cationic lipid analog material with a structure shown in formula (I):
- in formula (I), m1 is independently selected from the group consisting of a linear alkyl, a branched alkyl, phenyl, or a heteroatom-containing aryl;
- m2 is
- R1 is an alkyl, R2 is an alkyl, R3 is an alkyl or phenyl, or R2 and R3 are connected as a cyclic group or a heterocyclic group;
- m3 is independently selected from the group consisting of a linear alkyl, a linear alkenyl, or
- and
- m4 is independently selected from the group consisting of a linear alkyl, an ether bond-containing linear alkyl, or an N-heterocycle-containing alkyl.
Dependencies are inferred from the claim text.