# US 12,558,414 B2 · Method for vaccinating avians against reovirus

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- Number: US 12,558,414 B2
- Document ID: US12558414B2
- Status: Granted
- Kind: B2
- Application number: 17/626,340
- Filed: 2020-07-13
- Published: 2022-09-15
- Granted: 2026-02-24
- Earliest claimed: 2019-07-12
- Assignees: Biomune Company
- Applicants: Biomune Company
- Inventors: Kristi Mae Dorsey; Brianna Ford; Christopher Luther; John Knox Rosenberger
- Web page: https://patentgrep.com/patent/US12558414B2

## CPC

- A61K 39/12: Viral antigens
- A61K 39/21: Retroviridae, e.g. equine infectious anemia virus
- A61K 47/02: Inorganic compounds
- A61K 47/32: Macromolecular compounds obtained by reactions only involving carbon-to-carbon unsaturated bonds, e.g. carbomers {, poly(meth)acrylates, or polyvinyl pyrrolidone}
- A61K 2039/545: {characterised by the dose, timing or administration schedule}
- A61K 2039/552: {Veterinary vaccine}
- A61K 2039/55511: {Organic adjuvants}
- C12N 2720/12034: Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

The present invention relates to a method vaccinating avian against reovirus.

## Claims

### Claim 1

A method for vaccinating an avian against a reovirus or an associated disease, comprising administering to the avian a composition comprising at least one inactivated avian reovirus and an adjuvant comprising a lipophile and a polymer of acrylic or methacrylic acid, wherein the composition is administered to the avian between day 1 and day 14 of age.

### Claim 2 (depends on 1)

The method of claim 1, wherein the composition is administered to the avian between day 1 and day 7 of age.

### Claim 3 (depends on 2)

The method of claim 2, wherein the composition is administered to the avian within 24 hours post-hatching.

### Claim 4 (depends on 1)

The method of claim 1, wherein the composition comprises at least two distinct inactivated avian reoviruses.

### Claim 5 (depends on 1)

The method of claim 1, wherein the at least one inactivated avian reovirus is an avian pathogenic reovirus strain.

### Claim 6 (depends on 1)

The method of claim 1, wherein the inactivated reovirus is selected from inactivated strains S1, S2, S3, S1133, 2408, SS412, 2177, 1703, 1733, 1123, ERS-1 and 3005.

### Claim 7 (depends on 1)

The method of claim 1, wherein the composition comprises inactivated reovirus strains S1, S2 and S3.

### Claim 8 (depends on 1)

The method of claim 1, wherein the composition is administered at a dose from 103 TCID50 to 109 TCID50 of antigenic material per avian.

### Claim 9 (depends on 1)

The method of claim 1, wherein the lipophile is selected from the group consisting of medium chain EP triglycerides, medium chain triglycerides NF, medium chain fatty acid triglyceride JPE, caprylic/capric triglyceride, and combinations thereof.

### Claim 10 (depends on 1)

The method of claim 1, wherein the adjuvant further comprises at least one compound selected from saline, sterol, alcohol, saponin, sodium hydroxide, and any combination thereof.

### Claim 11 (depends on 1)

The method of claim 1, wherein the avian is a poultry.

### Claim 12 (depends on 1)

The method of claim 1, wherein a further administration with a composition comprising at least one avian reovirus antigenic material administered to the avian between 6 to 18 weeks of age.

### Claim 13 (depends on 12)

The method of claim 12, wherein the avian reovirus in the compositions is derived from a similar or a different avian reovirus strain.

### Claim 14

A method for inducing a protective immune response in an avian against at least one reovirus, comprising administering to said avian a vaccine comprising at least one antigenic material comprising at least one inactivated avian reovirus and an adjuvant comprising a lipophile and a polymer of acrylic or methacrylic acid, wherein the vaccine is administered to the avian between day 1 and day 14 of age.

### Claim 15 (depends on 11)

The method of claim 11, wherein the poultry is a chicken.

### Claim 16 (depends on 10)

The method of claim 10, wherein the sterol is cholesterol.

### Claim 17 (depends on 10)

The method of claim 10, wherein the alcohol is selected from ethanol, isopropanol, butanol and combinations thereof.