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US 12,558,381 B2Granted
Development of amnion-like tissue from human pluripotent stem cells
AssigneeThe Regents of the University of Michigan
InventorsJianping Fu, Deborah Gumucio, Yue Shao, Kenichiro Taniguchi, Yi Zheng, Sajedeh Nasr Esfahani
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Abstract
The present disclosure provides compositions and methods employing stem cell-derived amnion tissue. In some embodiments, compositions (e.g., scaffolds and devices) and methods of generating amnion-like tissues from hPSCs are provided. In some embodiments, uses of such cells for research, compound screening and analysis, and therapeutics are provided.
Claims9
A composition comprising an amnion-like tissue produced by a method, comprising:
- a) contacting cells selected from the group consisting of human induced pluripotent stem cells (hiPSCs) and human embryonic stem cells (hESCs) on a solid support coated with a gel matrix with a thickness of 100 μm, wherein the solid support comprises microposts with a height of greater than 3 μm;
- b) culturing said cells in a medium comprising Rho-kinase (ROCK) inhibitor Y-2763212 for 24 hours;
- c) coating said cells on said solid support with additional gel matrix such that said cells are surrounded by said gel matrix;
- d) replacing said Y-2763212 medium with a Y-2763212-free medium, and
- e) culturing said cells for at least 5 days under conditions such that amnion-like tissue is generated;
- wherein said amnion-like tissue comprises a squamous cyst comprising an epithelium, wherein said squamous cyst does not express NANOG, OCT4, or SOX2.
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Dependencies are inferred from the claim text.