Multiplex competition assay for profiling binding epitopes of affinity agents for clinical diagnostics use
AssigneeEnable Biosciences Inc.
InventorsCheng-Ting Tsai, Peter Robinson
Life of this patent
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Abstract
Some embodiments of the systems and methods provided herein relate to an assay. Some such embodiments include multiplex affinity probes and an antigen probe. multiplex affinity probes and an antigen probe Some embodiments include contacting a biological sample with the probes, wherein target binding agents such as antibodies in the biological sample compete away the multiplex affinity probes from binding to the antigen probe. Some such embodiments include detecting a decrease in binding of the multiplex affinity probes to the antigen probe, thereby indicating the presence or an amount of the target binding agents in the biological sample.
Claims17
An assay method, comprising:
- (a) providing a first multiplex affinity probe comprising a first affinity binding agent and a first DNA barcode probe;
- (b) providing a second multiplex affinity probe comprising a second affinity binding agent and a second DNA barcode probe;
- (c) providing an antigen probe comprising a first epitope recognizable by the first affinity binding agent, a second epitope recognizable by the second affinity binding agent, and a third DNA barcode probe;
- (d) contacting a biological sample with the antigen probe, wherein a first target antibody binds to the first epitope to form a first target antibody epitope complex if the biological sample comprises the first target antibody, and wherein a second target antibody binds to the second epitope to form a second target antibody epitope complex if the biological sample comprises the second target antibody;
- (e) contacting the biological sample with the first and second multiplex affinity probes, wherein the first multiplex affinity probe binds to the first epitope to form a first multiplex affinity probe epitope complex if the biological sample does not comprise the first target antibody, and wherein the second multiplex affinity probe binds to the second epitope to form a second multiplex affinity probe epitope complex if the biological sample does not comprise the second target antibody;
- and
- (f) detecting the presence or absence of the first and second multiplex affinity probe epitope complexes using a polymerase chain reaction (PCR),
- wherein the first and second affinity binding agent comprises an antibody or scFv thereof.
Dependencies are inferred from the claim text.