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US 12,553,891 B2Granted
Systems and methods for cyclic fluorescence imaging
AssigneeUniversity of Washington
InventorsDaniel T. Chiu, Chun-Ting Kuo, Li Wu
Life of this patent
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Abstract
Methods and systems for improved labeling and/or de-labeling a molecule or cell in the context of scientific experimentation, industrial applications, and clinical investigation, including the means to repeat the process of labeling and de-labeling in an efficient manner.
Claims16
A method of labeling and de-labeling a cell, the method comprising:
- providing a cell associated with a substrate;
- contacting the cell with a plurality of detectable agents, thereby labeling a plurality of sites of the cell with the plurality of detectable agents, wherein the plurality of detectable agents comprises a fluorescent detectable agent;
- contacting the labeled cell with a chemical signal quenching agent configured to quench fluorescence from the fluorescent detectable agent, thereby coupling the chemical signal quenching agent to the detectable agents and de-labeling the cell, wherein de-labeling comprises quenching of at least a portion of the plurality of detectable agents on the cell;
- and
- contacting the de-labeled cell with a second plurality of detectable agents, thereby re-labelling the cell, wherein the second plurality of detectable agents is different than plurality of detectable agents.
2 more elements
A method of de-labeling a labeled cell, the method comprising:
- providing a cell associated with a substrate, wherein the cell is labeled with a plurality of detectable agents including a fluorescent detectable agent;
- contacting the labeled cell with a chemical signal quenching agent configured to quench fluorescence from the fluorescent detectable agent thereby coupling the chemical signal quenching agent to the detectable agents and de-labeling at least 75% of sites labelled with the plurality of detectable agents on the labeled cell in an amount of time less than 15 minutes;
- and
- contacting the de-labeled cell with a second plurality of detectable agents, thereby re-labelling the cell, wherein the second plurality of detectable agents is different than plurality of detectable agents.
1 more element
Dependencies are inferred from the claim text.