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US 12,553,888 B2Granted
Fabrication of fluorescent nanoparticles and their conjugates for in vitro and in vivo diagnostics
AssigneeAuiset Biotechnology Co. Ltd.
InventorsNi Xie, Benzhong Tang
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Abstract
Provided are fluorescent nanoparticles and their conjugates and methods of using the same for in vivo and in vitro diagnostics and other applications. In some embodiments, provided are fluorescent nanoparticles with high solid-state absolute quantum yield. In some embodiments, provided are methods of manufacturing such nanoparticles. Nanoparticles may comprise monomers, such as styrene, and fluorophores, such as AlEgen™ Bright Green.
Claims18
A method of producing nanoparticles having a solid state luminescence quantum yield higher than 20%, the method comprising:
- a. forming a first solution having a luminophore concentration between 1 and 500 grams of luminophores per liter of the first solution by dissolving a first amount of luminophores in a second amount of one or more monomers;
- b. forming a second solution by dissolving a third amount of an initiator in a fourth amount of deionized water;
- c. forming a third solution by dissolving a fifth amount of a surfactant in a sixth amount of deionized water;
- d. forming a fourth solution by adding the first solution and the second solution to the third solution;
- and
- e. producing nanoparticles having the solid state luminescence quantum yield higher than 20% by heating the the fourth solution until the fourth solution is translucent white.
3 more elements
A method of producing nanoparticles having a solid state luminescence quantum yield higher than 20%, the method comprising:
- a. forming a first solution having a luminophore concentration between 1 and 500 gram of luminophores per liter of the first solution by dissolving a first amount of luminophores in a second amount of a monomer solution by adding the first amount of the luminophores to the second amount of the monomer solution;
- b. dividing the first solution into a first portion and a second portion;
- c. forming a second solution by dissolving a third amount of an initiator in a fourth amount of a deionized water by adding the third amount of the initiator in the fourth amount of the deionized water;
- d. dividing the second solution into a third portion and fourth portion;
- e. forming a third solution by dissolving a fifth amount of a surfactant in a sixth amount of the deionized water by adding the fifth amount of the surfactant in the sixth amount of the deionized water;
- f. dividing the third solution into a fifth portion and a sixth portion;
- g. forming a fourth solution by adding the first portion of the first solution and the third portion of the second solution to the fifth portion of the third solution;
- and
- h. producing a first plurality of nanoparticles having a first solid state luminescence quantum yield higher than 20% by heating the fourth solution until the fourth solution is translucent white;
- i. forming a fifth solution by adding the second portion of the first solution and the fourth portion of the second solution to the sixth portion of the third solution;
- j. stirring the fifth solution at a mechanical stirring speed having between 10 rotations per minute and 2000 rotations per minutes;
- and
- l. producing a second plurality nanoparticles having a second solid state luminescence quantum yield higher than 20% by heating the fifth solution until the fifth solution is translucent white.
10 more elements
A method of producing nanoparticles having a solid state luminescence quantum yield higher than 20%, the method comprising:
- a. forming a first solution having a luminophore concentration between 1 and 500 grams of luminophores per liter of the first solution by dissolving a first amount of luminophores in a second amount of a monomer solution by adding the first amount of the luminophores to the second amount of the monomer solution;
- b. dividing the first solution into a first portion and a second portion;
- c. forming a second solution by dissolving a third amount of an initiator in a fourth amount of a deionized water;
- d. dividing the second solution into a third portion and fourth portion;
- e. forming a third solution by dissolving a fifth amount of a surfactant in a sixth amount of the deionized water by adding the fifth amount of the surfactant in the sixth amount of the deionized water;
- f. dividing the third solution into a fifth portion and a sixth portion;
- g. forming a fourth solution by adding the first portion of the first solution and the third portion of the second solution to the fifth portion of the third solution;
- h. producing a first plurality of nanoparticles having a first solid state luminescence quantum yield higher than 20% by heating the fourth solution until the fourth solution is translucent white;
- i. forming a fifth solution by adding the second portion of the first solution and the fourth portion of the second solution to the sixth portion of the third solution
- j. performing an ultrasonic treatment to the fifth solution, the ultrasonic treatment having a power between 400 watts and 2000 watts of ultrasonic treatment;
- and
- l. producing a second plurality nanoparticles having a second solid state luminescence quantum yield higher than 20% by heating the fifth solution until the fifth solution is translucent white.
9 more elements
A method of producing nanoparticles having a solid state luminescence quantum yield higher than 20%, the method comprising:
- a. forming a first solution having a luminophore concentration between 1 and 500 grams of luminophores per liter of the first solution by dissolving a first amount of luminophores in a second amount of one or more monomers;
- b. dividing the first solution into a first portion and a second portion;
- c. forming a second solution by dissolving a third amount of an initiator in a fourth amount of deionized water;
- d. forming a third solution by dissolving a fifth amount of a surfactant in a sixth amount of deionized water;
- e. forming a fourth solution by adding the first portion of the first solution to the third solution and stirring the fourth solution at a mechanical stirring speed having between 10 rotations per minute and 1000 rotations per minute;
- f. forming a fifth solution by adding the second portion of the first solution and the second solution to the fourth solution;
- and
- g. producing nanoparticles having the solid state luminescence quantum yield higher than 20% by heating the fifth solution until the fifth solution is translucent white.
5 more elements
Dependencies are inferred from the claim text.