Methods, systems, and compositions for studying solvent accessibility and three-dimensional structure of biological molecules
AssigneeWisconsin Alumni Research Foundation
InventorsMichael R. Sussman, J. Leon Shohet, Faraz A. Choudhury, Joshua M. Blatz, Benjamin B. Minkoff, Daniel I. Benjamin
Life of this patent
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Abstract
This disclosure provides methods, systems, and compositions of matter for studying solvent accessibility and three-dimensional structure of biological molecules. A plasma can be used to generate marker radicals, which can interact with a biological molecule and mark the solvent-accessible portions of the biological molecule.
Claims20
A system for modifying a biological molecule, the system comprising:
- a sample chamber configured to contain a sample including a biological molecule, the sample chamber comprising a chemically and biologically inert inner surface;
- a plasma electrode;
- a power supply;
- and
- a control system in electronic communication with the power supply and the plasma electrode, wherein the control system is configured to cause the system to generate a plasma;
- wherein the plasma is generated such that, when the sample chamber contains the sample, one or more of a plurality of marker radical precursors is converted into one or more marker radicals to interact with the biological molecule,
- wherein the generating the plasma causes a detectable difference in the biological molecule without elevating a temperature of the sample by an amount that initiates denaturation of the biological molecule.
A system for modifying a biological molecule, the system comprising:
- a sample chamber configured to contain a sample including a biological molecule, the sample chamber comprising a chemically and biologically inert inner surface;
- a plasma electrode;
- a power supply;
- and
- a control system in electronic communication with the power supply and the plasma electrode, the control system configured to utilize electrical power from the power supply with the plasma electrode to generate a plasma;
- wherein the plasma is generated such that, when the sample chamber contains the sample, one or more of a plurality of marker radical precursors is converted into one or more marker radicals to interact with the biological molecule,
- wherein the generating the plasma causes a detectable difference in the biological molecule without elevating temperature of the sample by an amount that initiates denaturation of the biological molecule.
A system for modifying a biological molecule, the system comprising:
- a sample chamber configured to contain a sample including a biological molecule, the sample chamber comprising a chemically and biologically inert inner surface;
- a plasma electrode;
- a power supply;
- and
- a control system in electronic communication with the power supply and the plasma electrode, the control system configured to utilize electrical power from the power supply with the plasma electrode to generate a sequence of plasma pulses;
- wherein the sequence of plasma pulses is generated such that, when the sample chamber contains the sample, one or more of a plurality of marker radical precursors is converted into one or more marker radicals to interact with the biological molecule,
- wherein the generating the plasma causes detectable difference in the biological molecule without elevating temperature of the sample by an amount that initiates denaturation of the biological molecule.
Dependencies are inferred from the claim text.