Sensor with multiple reaction sites per pixel
AssigneeIllumina, Inc.
InventorsMohsen Rezaei, Michael Burek, Arvin Emadi, Ludovic Vincent
Life of this patent
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Abstract
There is set forth herein, in one example, an apparatus. The apparatus can comprise, for example: a first reaction site and a second reaction site over a single pixel. There is set forth herein, in one example, a method. The method can include, for example: detecting a signal emitted from a first reaction site and a second reaction site; determining the identity of a first analyte of interest in a first reaction site using an amplitude of the detected signal; and determining the identity of a second analyte of interest in a second reaction site using the amplitude of the detected signal.
Claims20
An apparatus comprising:
- a plurality of pixels;
- a first reaction site associated to a pixel of the plurality of pixels;
- a second reaction site associated to the pixel, wherein the first reaction site is defined by a first nanowell, and wherein the second reaction site is defined by a second nanowell;
- wherein the pixel includes a pixel sensor that detects a read signal, the read signal being dependent on a first cluster signal emitted from the first site and on a second cluster signal emitted from the second site;
- and
- wherein the first site and the second site are configured so that the second cluster signal in an “on” state has a greater amplitude than the first cluster signal in an “on” state.
An apparatus comprising:
- a plurality of pixels;
- a first reaction site associated to a pixel of the plurality of pixels;
- a second reaction site associated to the pixel;
- wherein the pixel includes a pixel sensor that detects a read signal, the read signal being dependent on a first cluster signal emitted from the first site and on a second cluster signal emitted from the second site;
- and
- wherein the first site and the second site are configured so that the second cluster signal in an “on” state has a greater amplitude than the first cluster signal in an “on” state, wherein the second reaction site is absent an attenuator aligned with and below a bottom surface of the second reaction site, and wherein the first reaction site includes an attenuator aligned with and below a bottom surface of the first reaction site so that the second cluster signal in the “on” state has a greater amplitude than the first cluster signal in the “on” state.
An apparatus comprising:
- a plurality of pixels;
- a first reaction site associated to a pixel of the plurality of pixels;
- a second reaction site associated to the pixel;
- wherein the pixel includes a pixel sensor that detects a read signal, the read signal being dependent on a first cluster signal emitted from the first site and on a second cluster signal emitted from the second site;
- and
- wherein the first site and the second site are configured so that the second cluster signal in an “on” state has a greater amplitude than the first cluster signal in an “on” state, and wherein, by an arrangement of attenuating material, attenuation of emission light rays radiating from the first reaction site is greater than attenuation of emission light rays radiating from the second reaction site so that the second cluster signal in the “on” state has a greater amplitude than the first cluster signal in the “on” state.
Dependencies are inferred from the claim text.