Methods of preserving a biological sample
Assignee10x Genomics, Inc.
InventorMohammad M. Shahjamali
Life of this patent
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Abstract
Provided herein are methods of preserving a biological sample on a first substrate, the methods including: (a) disposing a biological sample on a first substrate; (b) delivering a spacer agent to the first substrate, wherein the spacer agent is applied around the biological sample on the first substrate, thereby generating a chamber around the biological sample; (c) delivering a mounting agent to the chamber; and (d) assembling the first substrate with a second substrate, thereby preserving the biological sample on the first substrate.
Claims21
A method of preserving a biological sample on a first substrate, the method comprising:
- (a) disposing a biological sample on a first substrate;
- (b) delivering a mixture comprising a blocking paste and a plurality of beads to the first substrate, wherein the mixture comprising the blocking paste and the plurality of beads is applied around the biological sample on the first substrate, thereby generating a chamber around the biological sample, wherein the plurality of beads comprises beads approximately uniform in volume and/or shape;
- (c) delivering a mounting agent to the chamber;
- and
- (d) assembling the first substrate with a second substrate, thereby preserving the biological sample on the first substrate.
A method of preserving and analyzing a biological sample, the method comprising:
- (a) disposing a biological sample on a first substrate;
- (b) delivering a mixture comprising a blocking paste and a plurality of beads to the first substrate, wherein the mixture comprising the blocking paste and the plurality of beads is applied around the biological sample on the first substrate, thereby generating a chamber around the biological sample, wherein the plurality of beads comprises beads approximately uniform in volume and/or shape;
- (c) delivering a mounting agent to the biological sample within the chamber;
- (d) assembling the first substrate with a second substrate such that the chamber is sealed, thereby preserving the biological sample on the first substrate;
- (e) applying heat to the first substrate, thereby partially or completely melting the mixture comprising the blocking paste and the plurality of beads and removing the second substrate and the mixture comprising the blocking paste and the plurality of beads from the first substrate;
- (f) contacting the first substrate with an array comprising a plurality of capture probes, wherein a capture probe of the plurality of capture probes comprises:
- (i) a spatial barcode and (ii) a capture domain;
- and
- (g) performing spatial transcriptomic analysis.
Dependencies are inferred from the claim text.