Lamp primer set and method for amplifying nucleic acids using the same
OwnerFengying Shiinventor
Life of this patent
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Abstract
A LAMP primer set includes original LAMP primers of FIP, BIP, F3, and B3, and at least one autonomy primer. The original LAMP primers target regions F3, F2, F1C, B1C, B2, and B3 on nucleic acids, and the regions F3, F2, F1, B1C, B2C and B 3 C are located in order from 5′ end to 3′ end of a forward strand of the nucleic acids. The primer FIP includes oligonucleotides targeting F1C and F2, and the primer BIP includes oligonucleotides targeting B1C and B2. The at least one autonomy primer targets a region located beyond a region from F3 to B3.
Claims20
A LAMP primer set, comprising LAMP primers of FIP, BIP, F3, and B3, and at least one additional primer,
- wherein the LAMP primers of FIP, BIP, F3, and B3 target regions F3, F2, F1C, B1C, B2, and B3 on nucleic acids, and the regions F3, F2, F1, B1C, B2C, and B3C are located in order from 5′ end to 3′ end of a forward strand of the nucleic acids;
- wherein the primer FIP comprises oligonucleotides targeting the regions F1C and F2, and the primer BIP comprises oligonucleotides targeting the regions B1C and B2;
- and
- wherein the at least one additional primer targets a region located beyond-outside a region from F3 to B3.
A method for amplifying nucleic acids, comprising steps of:
- providing LAMP primers of FIP, BIP, F3, and B3, wherein the LAMP primers of BIP BIP, F3, and B3 target regions F3, F2, F1C, B1C, B2, and B3 on nucleic acids, and the regions F3, F2, F1, B1C, B2C, and B3C are located in order from 5′ end to 3′ end of a forward strand of the nucleic acids, wherein the primer FIP comprises oligonucleotides targeting the regions F1C and F2, and the primer BIP comprises oligonucleotides targeting the regions B1C and B2;
- providing at least one additional primer, wherein the at least one autonomy additional primer targets a region located outside a region from F3 to B3;
- and
- amplifying nucleic acids of a sample with the LAMP primers of FIP BIP F3, and B3 and the at least one additional primer.
Dependencies are inferred from the claim text.