Genes, constructs and maize event DP-202216-6
AssigneePioneer Hi-Bred International, Inc.
InventorsHeather Marie Christensen, Nathan David Coles, Olga Danilevskaya, Jeffrey Habben, Mary A Rupe, Jeffrey R Schussler, Bo Shen, Benjamin P Weers, Jingrui Wu
Life of this patent
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Abstract
The compositions and methods disclosed relate to DNA compositions, plant cells, seeds, plant parts that relate to maize plants with increased grain yield trait. Also provided are assays for detecting the presence of the maize DP-202216-6 event based on the DNA sequence of the recombinant construct inserted into the maize genome and the DNA sequences flanking the insertion site. Kits and conditions useful in conducting the assays are provided.
Claims12
A commodity product produced from a transgenic maize plant comprising event DP-202216-6, a representative sample of a seed comprising the event has been deposited with American Type Culture Collection (ATCC) under Accession No. PTA-124653, and comprising a recombinant DNA molecule comprising a nucleotide sequence selected from the group consisting of SEQ ID NOS:
- 7, 8, 9, 10, 11, 12, 13, 14, 31, and 32, and full complements thereof, wherein detection of said recombinant DNA molecule in a sample derived from said commodity product is determinative that said commodity product was produced from said transgenic maize plant comprising event DP-202216-6.
A method of increasing grain yield of a population of maize plants in a field, the method comprising growing a population of maize plants comprising event DP-202216-6, a representative sample of a seed comprising the event has been deposited with American Type Culture Collection (ATCC) under Accession No. PTA-124653, in a field and thereby increasing grain yield of the population of maize plants compared to a control plant not comprising the event DP-202216-6.
A recombinant DNA construct comprising an expression cassette, wherein the expression cassette in operable linkage comprises:
- a) a maize gos2 promoter;
- b) a maize ubiquitin gene 1 (ubiZM1) intron;
- c) a maize MADS box gene encoding a maize protein of SEQ ID NO:
- 1;
- d) a pinII terminator;
- e) a maize ubiquitin gene 1 (ubiZM1) promoter;
- f) a maize ubiquitin gene 1 (ubiZM1) 5′ UTR;
- g) a maize ubiquitin gene 1 (ubiZM1) intron;
- h) a mo-pat gene;
- and
- i) a pinII terminator.
Dependencies are inferred from the claim text.