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US 12,553,056 B2Granted
Modified polypeptide of meso-diaminopimelate dehydrogenase and polynucleotide encoding the modified polypeptide
AssigneeCj Cheiljedang Corporation
InventorsMina Baek, Seung-ju Son, Su Yon Kwon, Imsang Lee, Kwang Woo Lee
Life of this patent
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Abstract
The present disclosure relates to a modified polypeptide, in which the activity of meso-diaminopimelate is weakened, and a method for producing L-threonine using the same.
Claims11
A modified polypeptide, comprising the polypeptide of SEQ ID NO:
- 1, in which the amino acid corresponding to position 169 of the polypeptide of SEQ ID NO:
- 1 is substituted with leucine, and wherein said modified polypeptide has meso-diaminopimelate dehydrogenase activity.
A microorganism of the genus Corynebacterium, which comprises:
- a modified polypeptide, comprising the polypeptide of SEQ ID NO:
- 1, in which the amino acid corresponding to position 169 of the polypeptide of SEQ ID NO:
- 1 is substituted with leucine, and wherein said modified polypeptide has meso-diaminopimelate dehydrogenase activity;
- or a polynucleotide comprising the same.
1 more element
A method for preparing L-threonine, comprising a step of culturing in a medium a microorganism of the genus Corynebacterium comprising a modified polypeptide comprising the polypeptide of SEQ ID NO:
- 1, in which the amino acid corresponding to position 169 of the polypeptide of SEQ ID NO:
- 1 is substituted with leucine, wherein said modified polypeptide has meso-diaminopimelate dehydrogenase activity.
Dependencies are inferred from the claim text.