Auxin-inducible degron system kit and use thereof
AssigneesInter-University Research Institute Corporation Research Organization of Information and Systems, Kake Educational Institution
InventorsMasato Kanemaki, Ken-Ichiro Hayashi
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Abstract
An auxin-inducible degron system kit that controls degradation of a target protein in a non-plant-derived eukaryotic cell, the kit containing a first nucleic acid that encodes a mutant TIR1 family protein having a mutation at an auxin-binding site, an auxin analog that has an affinity to the mutant TIR1 family protein and a second nucleic acid that encodes a degradation tag containing at least a part of an Aux/IAA family protein and having an affinity to a complex of the mutant TIR1 family protein and the auxin analog.
Claims5
An auxin-inducible degron system kit that controls degradation of a target protein in a mammalian cell, the kit comprising:
- a first nucleic acid that encodes a mutant F-box transport inhibitor response 1 (TIR1) family protein having a mutation at an auxin-binding site;
- an auxin analog having an affinity to the mutant TIR1 family protein;
- a second nucleic acid that encodes a degradation tag including at least a part of an Auxin/Indole acetic acid (Aux/IAA) family protein and having an affinity to a complex of the mutant TIR1 family protein and the auxin analog, wherein the mutant TIR1 family protein is a protein in which an F74 of Oryza sativa TIR1 (OsTIR1) has mutated into A, G or S;
- and
- a third nucleic acid that encodes the target protein linked upstream or downstream of the second nucleic acid,
- wherein the at least part of the Aux/IAA family protein is Mini-auxin-inducible degron (mAID) of which the amino acid sequence is SEQ ID NO. 8,
- and wherein the auxin analog is a compound of the following general formula (I-1) or an ester body thereof,
- wherein in the general formula (I-1), R1 is an alkyl group having 1 to 6 carbon atoms, an alkoxy group having 1 to 6 carbon atoms, or a halogen atom, n is an integer of 0 to 5, and, in a case where n is an integer of 2 to 5, n R1s are identical to or different from each other.
Dependencies are inferred from the claim text.