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US 12,553,024 B2Granted
Serum-free media formulation for culturing cells and methods of use thereof
AssigneeJuno Therapeutics, Inc.
InventorsPascal Beauchesne, Kien Khuu-Duong, Ivie Aifuwa, Calvin Chan
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Abstract
Provided herein is a serum-free media for culturing, such as cultivating, preparing and/or producing cells, such as immune cells, such as genetically engineered cells. Also provided is a liquid basal media and frozen supplements that can be used to produce serum-free media. The provided embodiments include methods for producing serum-free media and methods for culturing cells, such as activating, transducing, cultivating or expanded cells, in the presence of serum-free media.
Claims13
A serum-free medium for use for culturing T cells, comprising:
- (a) 1 mM to 3 mM of L-alanyl-L-glutamine;
- (b) 1 mM to 3 mM of L-glutamine;
- (c) 100 IU/mL to 200 IU/mL of IL-2;
- (d) 500 IU/mL to 1400 IU/mL of IL-7;
- (e) 100 IU/mL to 200 IU/mL of IL-15;
- (f) at least one protein, wherein the at least one protein comprises human serum albumin;
- and
- (g) a base medium;
- wherein the serum-free medium is free of serum.
6 more elements
Dependencies are inferred from the claim text.