Method for separation of antibodies or antibody fragments being devoid of an Fc region capable of binding to protein A

A method for separation of antibodies or antibody fragments, comprising the steps of:

• a) providing a feed comprising antibodies or antibody fragments capable of binding to Protein A, where the antibodies or antibody fragments comprise a VH3 region and are devoid of an Fc region capable of binding to Protein A;
• b) contacting said feed with a separation resin having ligands covalently coupled to a support, wherein said ligands consist essentially of a polypeptide as defined by SEQ ID NO 1:
• AQX1AFYEILX2LPNLTEEQRX3AFIQSLKDDPSVSKAILAEAKKLNX4
• AQ
• wherein:
• X1=E, K, Y, T, F, L, W, I, M, V, A, H or R,
• X2=H or K,
• X3=N or A, and
• X4=D, F, Y, W, K or R
• and wherein said antibodies or antibody fragments bind to said separation resin;
• c) optionally washing said separation resin with a washing liquid;
• and
• d) eluting said antibodies or antibody fragments from said separation resin with an elution liquid and recovering said antibodies or antibody fragments.