Chimeric antigen receptor t lymphocyte for treating tumors, preparation method therefor, and use thereof

A method of treatment or adjuvant treatment of a Siglec-15-positive tumor comprising administering to the subject in need of such treatment an effective amount of CAR (chimeric antigen receptor)-T cells comprising

• an isolated nucleic acid molecule encoding a chimeric antigen receptor successively comprising:
• a single chain antibody fragment (scFv) against the tumor associated antigen Siglec-15,
• a human CD8 transmembrane region,
• a human intracellular signaling domain,
• a P2A self-cleaving peptide and
• a full-length CD27,
• wherein, the human intracellular signaling domain includes an intracellular costimulatory signal domain of human 4-1BB and a signal transduction domain of human CD3ζ;
• the amino acid sequence of the chimeric antigen receptor is shown in SEQ ID NO. 2;
• the Siglec-15-positive tumor is a human glioma tumor;
• and
• the treatment reduces tumor volume.