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US 12,552,842 B2Granted
Alkaliphilic consortium shifting for production of phycocyanins and biochemicals
AssigneeSynergia Biotech Inc.
InventorsAgasteswar Vadlamani, Cigdem Demirkaya, Jacqueline Zorz, Hector De La Hoz Siegler, Marc Strous
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Abstract
Processes are disclosed for photosynthetic cyanobacterial production of selected proteins and biochemicals within an evolving alkaliphilic microbial consortium.
Claims19
A method of making a phycocyanin product, comprising:
- culturing an alkaliphilic soda lake microbial consortium under aerobic alkaline conditions in a diurnal growth cycle to establish a cyanobacterial population containing the phycocyanin within the microbial consortium in a cultured biomass in an aerobic culture comprising a growth medium comprising from 0.25-3M Na+;
- and,
- shifting the microbial consortium, having a proportion of cyanobacteria and a proportion of non-cyanobacterial alkaliphile microbes, to a dark-phase cycle under anaerobic alkaline conditions in an anaerobic culture comprising from 0.25-3M Na+ to initiate auto-catabolic release of the phycocyanin intact from the cyanobacterial population as the proportion of cyanobacteria in the microbial consortium decreases and the proportion of non-cyanobacterial alkaliphile microbes increases in the consortium, to produce a biomass solids product and an aqueous phycocyanin product.
Dependencies are inferred from the claim text.