Method for extracting microvesicles from biological sample

A method for extracting microvesicles from a biological sample, the method comprising:

• adding a polyvalent cationic substance to the biological sample to form multiple aggregates in which the microvesicles and the polyvalent cationic substance are aggregated with other via an electrical force;
• passing the biological sample containing the aggregates through a capture filter while the aggregates are captured by the capture filter;
• passing a buffer solution having a negative charge through the capture filter in which the aggregates are captured such that the aggregates are released from the capture filter;
• and injecting an elution solution into the aggregates released from the capture filter such that the microvesicles are separated from the aggregates, and then extracting the separated microvesicles, wherein the capture filter comprises a hydrophilic material selected from the group consisting of sliver metal, polyethersulfone, glass fiber, polycarbonate track etch (PCTE), and mixed cellulose esters, and wherein the polyvalent cationic substance comprises polyarginine, poly histidine, cationic dendrimer, poly amidoamine, polyquaternium, or a combination thereof.