Fusion antibody for presenting antigen-derived T cell antigen epitope or peptide containing same on cell surface, and composition comprising same
AssigneeAjou University Industry-Academic Cooperation Foundation
InventorsYong Sung Kim, Jeong-Ah Kim, Keunok Jung, Seyoung Lee, Min-Jeong Son
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Abstract
The present invention relates to: a composition for delivering a viral antigen-derived CD8+ T cell antigen epitope or a peptide comprising same to the cytoplasm of a target cell to thereby present the epitope or peptide to major histocompatibility complex class I (MAC-1), which is an antigen-presenting molecule on the cell surface; a composition comprising same; and a use thereof.
Claims22
A fusion antibody in which a peptide comprising a CD8+ T-cell antigen epitope comprising a sequence selected from the group consisting of SEQ ID NOs:
- 13 to 16, 18, 19, 21 to 24, 26 to 33, 35 and 36 is fused to a cell- or tissue-specific cytosol-penetrating antibody, wherein the cytosol-penetrating antibody comprises a heavy chain comprising the sequence of SEQ ID NO:
- 1 and a light chain comprising the sequence of SEQ ID NO:
- 2,
- wherein the fusion antibody is endocytosed into a cell by the cytosol-penetrating antibody and is then localized in a cytosol through endosomal escape.
A method of presenting a CD8+ T-cell antigen epitope on a surface of a target cell, in which a peptide comprising the CD8+ T-cell antigen epitope is delivered into a cytosol in a form of a fusion antibody in which the peptide comprising the CD8+ T-cell antigen epitope comprising a sequence selected from the group consisting of SEQ ID Nos. 13 to 16, 18, 19, 21 to 24, 26 to 33, 35 and 36 is fused to a cell- or tissue-specific cytosol-penetrating antibody, wherein the cytosol-penetrating antibody comprises a heavy chain comprising the sequence of SEQ ID No:
- 1 and a light chain comprising the sequence of SEQ ID No:
- 2, and wherein the fusion antibody is endocytosed into the cell and then localized in the cytosol through endosomal escape, whereby the CD8 T-cell antigen epitope is processed and then presented on the surface of the target cell.
Dependencies are inferred from the claim text.