Recombinant nucleic acids containing alphaherpesvirus promoter sequences
AssigneeThe Trustees of Princeton University
InventorsEsteban Engel, Carola Maturana, Lynn Enquist
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Abstract
Provided are recombinant nucleic acids (e.g., vectors), and related methods, for expression of a target gene in a host cell. The recombinant nucleic acids comprise a promoter comprising a nucleic acid sequence having at least 70% sequence identity to a sequence selected from SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8 and SEQ ID NO: 9; a cloning site for insertion of a nucleic acid encoding the target gene; and at least one non-promoter regulatory element required for the expression of the target gene in the host cell.
Claims18
A recombinant vector, comprising:
- a promoter between about 200 bp and about 410 bp in length, wherein the promoter comprises a nucleic acid sequence having 100% sequence identity to SEQ ID NO:
- 2;
- a cloning site for insertion of a nucleic acid encoding the target gene;
- and
- at least one non-promoter regulatory element required for the expression of the target gene in the host cell.
Dependencies are inferred from the claim text.