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US 12,551,558 B2Granted
Compositions and methods for promoting hematopoietic cell cytotoxicity
AssigneesEmory University, Children's Healthcare of Atlanta, Inc.
InventorsHarold Trent Spencer, Christopher Doering, Shanmuganathan Chandrakasan, Lauren Fleischer, Jaquelyn Zoine, Gianna Branella, Harrison C. Brown
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Abstract
Provided herein are compositions and methods for promoting hematopoietic cell cytotoxicity.
Claims17
A method for treating leukemia comprising:
- (a) introducing into cells obtained from a first subject a nucleic acid construct comprising a nucleic acid sequence encoding a recombinant protein comprising thrombopoietin (TPO) linked to a CH3 hinge,
- a transmembrane domain,
- and CD3-zeta signaling domain, providing cells that express the recombinant protein;
- wherein the thrombopoietin (TPO) linked to a CH3 hinge is encoded by a codon optimized nucleic acid having the nucleic acid sequence of SEQ ID NO:
- 15;
- and
- (b) administering an effective amount of the cells that express the recombinant protein to a second subject diagnosed with leukemia;
- wherein the cells are selected from the group consisting of:
- alpha beta T cells, cytotoxic T lymphocytes, T helper cells, lymphokine-activated cells, tumor-infiltrating lymphocytes, NK cells, nave T cells, memory T cells, gamma delta T cells, NKT cells, and macrophages.
6 more elements
A vector comprising a nucleic acid sequence encoding a recombinant protein comprising thrombopoietin (TPO) linked to a CH3 hinge encoded by a codon optimized nucleic acid having the nucleic acid sequence of SEQ ID NO:
- 15.
Dependencies are inferred from the claim text.