African swine fever virus chimeric protein, vaccine composition, preparation method and use thereof

An African swine fever virus chimeric protein comprising:

• a. African swine fever virus p72 domain I, with an amino acid sequence as shown in SEQ ID NO.2;
• b. African swine fever virus p72 domain II, with an amino acid sequence as shown in SEQ ID NO.3;
• c. African swine fever virus p72 domain III, with an amino acid sequence as shown in SEQ ID NO. 4;
• and
• d. African swine fever virus antigenic protein;
• e. the African swine fever virus p72 domain I is located at the N-terminus of the chimeric protein, the African swine fever virus p72 domain III is located at the C-terminus of the chimeric protein, and the African swine fever virus p72 domain II is located between the domain I and the domain III, the African swine fever virus antigenic protein is located between the domain I and the domain II or between the domain II and the domain III;
• f. the African swine fever virus antigenic protein is one fragment of p54, p30, CD2v and p12 proteins or one of the fragments as shown in SEQ ID NOs. 38-58;
• g. the fragment of p54 protein is selected from any one of SEQ ID NOs. 5, 6, 7, 8, 9, 10, 11, and 12;
• the fragment of p30 protein is selected from any one of SEQ ID NOs. 13, 14, 15, 16, 17, 18, and 19;
• the fragment of CD2v protein is selected from any one of SEQ ID NOs. 20, 21, 22, 23, 24, 25, 26, 27, 28, 29 and 30;
• and
• the fragment of p12 protein is selected from any one of SEQ ID NOs. 31, 32, 33, 34, 35, 36, and 37.