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US 12,551,506 B2Granted
Differentiation of olfactory mucosa derived mesenchymal stem cells to schwann cells for peripheral nerve repair
AssigneeNortheastern University
InventorsRyan A. Koppes, Katelyn E. Neuman, Abigail N. Koppes, Aidan Kenny
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Abstract
Provided herein are, in various embodiments, methods and compositions for differentiating olfactory mucosa-derived mesenchymal stem cells (OM-MSC). In certain embodiments, the disclosure provides for media to differentiate OM-MSCs. In still further embodiments, the disclosure provides for methods and compositions using differentiated OM-MSCs for the treatment of nerve repair. In particular embodiments, the disclosure provides for novel treatments of peripheral nerve repair.
Claims10
A method of promoting neurite outgrowth in a neuron, the method comprising:
- a) propagating olfactory mucosa-derived mesenchymal stem cells (OM-MSCs) in Schwann cell conditioned media (SCCM) for about 14-21 days, thereby producing differentiated olfactory mucosa-derived mesenchymal stem cells (dOM-MSCs);
- b) isolating the-dOM-MSCs, thereby producing isolated dOM-MSCs;
- and
- c) contacting the neuron with a composition comprising the isolated dOM-MSCs, thereby promoting neurite outgrowth in the neuron.
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Dependencies are inferred from the claim text.