Lens-free holographic optical system for high sensitivity label-free microbial growth detection and quantification for screening, identification, and susceptibility testing
AssigneeAST Revolution, LLC
InventorOleg Gusyatin
Life of this patent
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Abstract
Disclosed are optical interrogation apparatus that can produce lens-free images using an optoelectronic sensor array to generate a holographic image of sample objects, such as microorganisms in a sample. Also disclosed are methods of detecting and/or identifying microorganisms in a biological sample, such as microorganisms present in low levels. Also disclosed are methods of using systems to detect microorganisms in a biological sample, such as microorganisms present in low levels. In addition or as an alternative, the methods of using systems may identify microorganisms present in a sample and/or determine antimicrobial susceptibility of such microorganisms.
Claims16
A method for detecting a cell in a sample, comprising:
- a. detecting a first in-line hologram of at least one object in the sample;
- b. measuring a variation over time of the in-line hologram;
- c. determining the presence or absence of phenotypical behavior or event for the at least one object in the sample based on the measured variation over time of the in-line hologram thereby detecting at least one cell in the sample;
- d. immobilizing the at least one object in the sample, wherein immobilizing the at least one object in the sample comprises allowing the at least one object in the sample to settle onto a surface in a reaction chamber or suspending the at least one object in the sample in a porous medium;
- and
- e. subjecting the porous medium to a phase change to immobilize the at least one object in the sample in a three-dimensional space.
A method for detecting the presence of a living cell in a sample, comprising:
- a. detecting a first in-line hologram of at least one object in the sample;
- b. detecting a second in-line hologram of at least one object in the sample;
- c. measuring a variation over time between the first and second in-line holograms;
- and
- d. associating the variation over time with a living cell thereby detecting the presence of a living cell in the sample;
- wherein the sample includes sample volume portions that are present in a plurality of respective chambers comprising differential growth media.
The method of 8, further comprising producing an output corresponding to (1) identification, (ii) morphology, (iii) movement, and/or phenotypic classification of the at least one object in the sample based on a trained set of network layers.
Dependencies are inferred from the claim text.