Augmented-field-of-view, high-resolution optical microscopy method and optical microscope

An optical microscope (100) having a microscope body and a housing (10) surrounding the microscope body, the optical microscope comprising a sample holder (11) adapted to receive a sample (1) in an object plane (3), a microscope objective (21, 22, 23) adapted to collect an image beam (30) from an object field in the object plane (3) and to form an intermediate image of the object field in an image plane (33), the optical microscope having a Fourier plane (71) located between the object plane (3) and the image plane (33) inside the microscope housing, wherein the housing includes an output port, and the optical microscope includes an imaging module (200) arranged outside the microscope housing and a controller (300), the imaging module (200) comprising a first camera (51), a second camera (52), an optical beam splitter (40) adapted to receive the image beam (30) of the image plane (33) and to form a first image beam (31) and a second image beam (32), a first optical system (41, 42, 43) arranged between the beam splitter (40) and the first camera (51), a second optical system (44, 45, 46, 47) arranged between the beam splitter (40) and the second camera (52), the second optical system (44, 45, 46, 47) and the second camera (52) being configured to acquire a wide-view image capable of extending over the entire object field of the microscope objective (21, 22, 23), the first optical system (41, 42, 43) having a higher magnification than the second optical system (44, 45, 46, 47) and the first optical system (41, 42, 43) comprising an offset optical system (41) arranged to offset the Fourier plane (71) via the output port outside the housing and to form the image of the Fourier plane (71) in an image Fourier plane (72), the first optical system (41, 42, 43) including a reflective scanning device (42, 48) arranged in or near the image Fourier plane (72), optically conjugate with the Fourier plane (71) of the optical microscope (100) and the first optical system (41, 42, 43) including a lens (43), the first camera (51) being located in the focal plane of the lens (43), the controller (300) being adapted to angularly orient the reflective scanning device (42, 48) in such a way that the first camera (51) acquires at least one first image (61, 62, . . . , 6N) of a portion of the object field of the microscope objective (21, 22, 23), simultaneously with the wide-view image of the object field acquired by the second camera (52), and the controller (300) being adapted to angularly orient the angular reflective scanning device (42, 48) in a series of at least two positions, the first camera (51) being adapted to acquire a series of first images (61, 62, . . . , 6N) of at least two portions of the object field without moving the sample, without changing the microscope objective and without changing the optical magnification between the object field and the first camera (51), the microscope (100) comprising an image processing system adapted to receive the series of first images (61, 62, . . . , 6N) and to reconstruct a high-resolution mosaic image (600) of the object field.