Flow cytometers including sample injection needles, and methods of use thereof

Abstract

Flow cytometers having sample injection needles are provided. The subject flow cytometers include a flow cell for transporting particles in a core stream of a flow stream from a proximal end to a distal end, and a sample injection needle having a passage running therethrough for delivering sample fluid from a sample injection line at a proximal end to the flow cell at a distal end to generate the core stream. Flow cells of interest include a flow cell cone at the proximal end, and sample injection needles of interest are configured and positioned to maintain an intact core stream under flow conditions that vary by a magnitude or more. Methods of assembling the subject flow cytometers and methods of analyzing a sample using the subject flow cytometers are also provided.

Dependencies are inferred from the claim text.