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US 12,546,787 B2Granted
Non-invasive paper based device for pregnancy detection in cattle and buffaloes
AssigneeCouncil of Scientific and Industrial Research
InventorsGovindhaswamy Umapathy, Amit Asthana, Mohan Rao Chintalagiri, Vinod Kumar, Suresh Gopi
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Abstract
The present invention discloses a kit and a novel method of extraction of a progesterone metabolite from feces/dung sample. The present invention also discloses a paper-based microfluidic device for detection of the progesterone metabolite for early determination of pregnancy in cattle and buffaloes. The invention further discloses antibodies developed against said metabolite, method of extraction, methods for fabricating the paper-based microfluidic devices and methods for inexpensive, rapid and non-invasive determination of pregnancy in early stages in cattle and buffaloes.
Claims12
A microfluidic device comprising:
- a first layer comprising:
- a first hydrophilic material layer,
- a first hydrophobic material layer disposed on a surface of the first hydrophilic material layer,
- a sample port for receiving a biological sample, and
- a reagent port for receiving a reagent;
- a second layer adjacent the first layer and comprising:
- a second hydrophilic material layer,
- a second hydrophobic material layer disposed on a surface of the second layer,
- a first circular zone for receiving the biological sample from the sample port and delivering the biological sample one or more antibody-enzyme complex storage zones, and
- a second circular zone for receiving the reagent from the reagent port and delivering the reagent to the one or more antibody-enzyme complex storage zones,
- where the second hydrophobic material layer is configured to face the first layer and the first hydrophobic material layer is configured to face away from the second layer;
- a third layer adjacent the second layer and comprising:
- a third hydrophilic material,
- a third hydrophobic material layer disposed on a surface of the third layer, and
- one or more antibody-enzyme complex storage zones,
- wherein the third hydrophobic material layer is configured to face the second layer;
- and
- a fourth layer adjacent the third layer and comprising:
- a fourth hydrophilic material layer,
- a fourth hydrophobic material layer disposed on a surface of the fourth layer, and
- a test zone configured for fluidic communication with the one or more antibody-enzyme complex storage zones and comprising immobilized 5α-pregnan-3α-ol-20-one antigen for colorimetric detection of 5α-pregnan-3α-ol-20-one,
- where the fourth hydrophobic material layer is configured to face the third layer,
- wherein the second layer further comprises an inverted y-shaped microfluidic channel for receiving reagent from the reagent port at a center portion of the y-shaped microfluidic channel and delivering the reagent to the second circular zone, a third circular zone, and a fourth circular zone, where the second circular zone is configured to deliver the reagent to the one or more antibody-enzyme complex storage zones, where the third circular zone configured to deliver the reagent to a positive control zone of the fourth layer, and where the fourth circular zone is configured to deliver the reagent to a negative control zone of the fourth layer.
20 more elements
A microfluidic device comprising:
- a first layer comprising:
- a first hydrophilic material layer,
- a first hydrophobic material layer disposed on a surface of the first hydrophilic material layer,
- a sample port for receiving a biological sample, and
- a reagent port for receiving a reagent;
- a second layer adjacent the first layer and comprising:
- a second hydrophilic material layer,
- a second hydrophobic material layer disposed on a surface of the second layer,
- a first circular zone for receiving the biological sample from the sample port and delivering the biological sample one or more antibody-enzyme complex storage zones, and
- a second circular zone for receiving the reagent from the reagent port and delivering the reagent to the one or more antibody-enzyme complex storage zones,
- where the second hydrophobic material layer is configured to face the first layer and the first hydrophobic material layer is configured to face away from the second layer;
- a third layer adjacent the second layer and comprising:
- a third hydrophilic material,
- a third hydrophobic material layer disposed on a surface of the third layer, and
- the one or more antibody-enzyme complex storage zones,
- wherein the third hydrophobic material layer is configured to face the second layer;
- and
- a fourth layer adjacent the third layer and comprising:
- a fourth hydrophilic material layer,
- a fourth hydrophobic material layer disposed on a surface of the fourth layer, and
- a test zone configured for fluidic communication with the one or more antibody-enzyme complex storage zones and comprising immobilized 5α-pregnan-3α-ol-20-one antigen for colorimetric detection of 5α-pregnan-3α-ol-20-one,
- where the fourth hydrophobic material layer is configured to face the third layer,
- wherein
- the first layer, the second layer, the third layer and the fourth layer are connected in series and linearly in the form of a sheet with the first layer and the fourth layer at ends of the sheet;
- and
- the linear sheet is foldable into a vertical stack to form the microfluidic device, the vertical stack in the order of the first layer, the second layer, the third layer and the fourth layer with the first layer and the fourth layer at ends of the vertical stack.
23 more elements
Dependencies are inferred from the claim text.