Methods and compositions for amplified electrochemiluminescence detection assays
AssigneeVibrant Holding, LLC
InventorsJohn J. Rajasekaran, Vasanth Jayaraman, Tianhao Wang, Kang Bei, Hari Krishnan Krishnamurthy
Life of this patent
Estimating the term…
Abstract
Disclosed herein are formulations, substrates, and arrays. Also disclosed herein are methods for manufacturing and using the formulations, substrates, and arrays. Also disclosed are methods for identifying peptide sequences useful for diagnosis and treatment of disorders, and methods for using the peptide sequences for diagnosis and treatment of disorders, e.g., celiac disorder. In certain embodiments, substrates and arrays comprise a porous layer for synthesis and attachment of polymers or biomolecules.
Claims10
An AECL tag composition, comprising:
An assay plate for mounting a solid state microarray,
- said assay plate comprising:
- a pillar, said pillar comprising a top surface and a bottom surface, said top surface comprising a mounting surface to receive a solid state microarray and at least one working electrode and one counter electrode configured to contact and to be in electrical communication with a corresponding at least one working electrode and counter electrode on a bottom surface of said solid state microarray, said bottom surface of said pillar comprising contacts for supplying power to said at least one pillar working electrode and counter electrode,
- wherein said solid state microarray comprises an addressable network of working electrodes and counter electrodes integral to said microarray, wherein said working electrodes comprise chemically-functionalized surfaces comprising functional groups, said functional groups capable of covalently binding capture ligands at defined locations on a substrate, and said capture ligands capable of specifically binding said target biomolecules thereby immobilizing said target biomolecules at said defined locations on said substrate.
Dependencies are inferred from the claim text.