Cartridge for processing biological samples and devices and methods thereof

A cartridge for dissociating and obtaining separated individual cells from a biological sample, the cartridge comprising:

• (a) a sample processing unit comprising:
• a sealable port with a removable cap configured to receive the biological sample for introduction to the sample processing unit;
• a sample chamber configured to collect the biological sample;
• and
• a mincer,
• wherein the mincer is configured to interface with the sample chamber;
• (b) a reaction unit comprising a reaction chamber configured to receive ultrasonic energy via a temperature-controlled, fluid coupling medium from a transducer assembly located outside of the cartridge in an ultrasonic processing device,
• wherein the sample processing unit and the reaction unit are connected via a first channel;
• (c) a filtration unit comprising:
• a filtration chamber;
• and
• a filtration device comprising a tubular assembly with a cell filter covering a bottom opening of the tubular -assembly, wherein the tubular assembly is configured to enter the filtration chamber,
• wherein the reaction chamber and the filtration unit are connected via a second channel;
• and
• (d) a collection unit configured to receive the separated individual cells from the biological sample,
• wherein the filtration unit and the collection unit are connected via a third channel;
• (e) one or more gates,
• wherein each gate is configured to control a fluidic connection between any one or more of:
• the sample processing unit and the reaction unit via the first channel;
• the reaction unit and the filtration unit via the second channel;
• and the filtration unit and the collection unit via the third channel,
• wherein the cartridge is configured to be loadable into the ultrasonic processing device.