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US 12,545,940 B2Granted
Method for producing a deuterated or tritiated NAD(P)H
AssigneeOxford University Innovation Limited
InventorsKylie Vincent, Holly Reeve, Jack Rowbotham
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Abstract
Described herein is a method of producing a reduced labelled cofactor comprising one or more xH atom, wherein x is 2 or 3. Described herein is also a method of producing a reduced labelled reaction product comprising one or more xH atom, wherein x is 2 or 3, wherein the method comprises producing a reduced labelled cofactor according to the invention. Described herein are also systems for performing such methods.
Claims28
A method of producing a reduced labelled cofactor comprising one or more xH atoms, wherein x is 2 or 3, wherein said method comprises:
- (i) providing a composition comprising xH+ ions and an oxidised cofactor;
- (ii) transferring electrons from an electron source to a first polypeptide, which is an NADH:acceptor oxidoreductase or an NADPH:acceptor oxidoreductase;
- and
- (iii) contacting the xH+ ions and the oxidised cofactor with the first polypeptide, thereby reducing the oxidised cofactor to form a reduced labelled cofactor comprising one or more xH+ atoms.
1 more element
Dependencies are inferred from the claim text.