Back to results
US 12,545,897 B2Granted
Engineered intestinal tissue and uses thereof
AssigneeOrganovo, Inc.
InventorsKelsey Nicole Retting, Deborah Lynn Greene Nguyen, Lauran Madden
Life of this patent
Estimating the term…
Abstract
Disclosed are methods of assessing the ability of a candidate therapeutic agent to reverse, reduce or prevent intestinal injury by a potential toxic agent using a three-dimensional, engineered, bioprinted, biological intestinal tissue model. Also disclosed are methods of assessing the effect of an agent on intestinal function, the method comprising contacting the agent with a three-dimensional, engineered, bioprinted, biological intestinal tissue model.
Claims24
A method of making an intestinal tissue model, the method comprising:
- a. depositing a layer comprising adult primary intestinal myofibroblasts at a density of at least about 5×106 cells per milliliter onto a biocompatible surface;
- and
- b. depositing a layer comprising adult primary intestinal epithelial cells onto and in contact with the apical surface of the layer comprising adult primary intestinal myofibroblasts to produce the intestinal tissue model.
Dependencies are inferred from the claim text.