Compositions and methods for derivation of trophoblast stem cells
AssigneeNorth Carolina State University
InventorsBalaji Rao, Adam Mischler
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Abstract
The present disclosure provides compositions and methods related to the derivation of trophoblast stem cells. In particular, the present disclosure provides novel formulations and methods for deriving and maintaining trophoblast stem cells from human pluripotent stem cells, which can be used for basic research purposes as well as for developing treatments for pregnancy-associated pathologies such as preeclampsia, recurrent loss of pregnancy, placenta accreta, and intrauterine growth restriction.
Claims10
A method for inducing differentiation of human embryonic stem cells (hESCs) into cytotrophoblasts (CTBs) or CTB-like cells, the method comprising:
- culturing the hESCs in a chemically defined cell culture medium comprising a sphingosine 1-phosphate receptor (SIPR) agonist, an activin/nodal inhibitor, and at least one growth factor;
- and
- assessing the hESCs for one or more phenotypic characteristics of CTBs or CTB-like cells.
Dependencies are inferred from the claim text.