Bioprocess device assembly and inoculation method

A bioprocess device assembly, comprising:

• at least a first bioreactor and a second bioreactor, wherein the first bioreactor is a rocking motion bioreactor,
• a transfer system for transferring a certain volume of a medium contained in the first bioreactor into the second bioreactor,
• a first biomass sensor associated with the first bioreactor for measuring at least one parameter relating to a quantity and/or a quality of biomass contained in the first bioreactor,
• a second biomass sensor associated with the second bioreactor, wherein the second biomass sensor is configured to examine the transferred medium, and wherein the second biomass sensor is a single-frequency capacitance sensor, a scattered light sensor, and/or a light intensity sensor, and
• a control unit configured for receiving measurement data from the first biomass sensor and controlling the transfer system,
• the control unit being parametrized such that, based on an evaluation of the measurement data received from the first biomass sensor, the control unit:
• (a) decides whether a biomass status is sufficient for inoculation,
• (b) determines a necessary inoculation volume, and
• (c) controls the transfer system to transfer the determined inoculation volume from the first bioreactor into the second bioreactor.