Systems and apparatuses for cellular therapeutics manufacture

A cartridge for manufacturing a population of cells, comprising:

• a sealed enclosure with an inlet port and an outlet port, comprising:
• a first fluidic network connected to the outlet port;
• a first reagent reservoir connected to the first fluidic network;
• a first cell analysis region connected to the first fluidic network;
• and
• a chamber for culturing cells, wherein the chamber comprises:
• a first input opening for introduction of fluid into the chamber;
• a first output opening for removal of fluid from the chamber;
• and
• a second output opening for removal of fluid from the chamber;
• wherein:
• the chamber is connected to each of the outlet port, the first reagent reservoir, and the first cell analysis region via the first fluidic network;
• the first and second output openings are positioned at different vertical elevations within the chamber;
• and
• an internal surface of a base of the chamber comprises a plurality of concave features defined thereon.