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US 12,545,860 B2Granted
Mannanase variants
AssigneeBASF SE
InventorsJesper Nielsen, Katie Kline, Amanda Rae Logue, Cindy Hoang, Mark Miller, Asfia Qureshi, Tong Li, Priya Anand
Life of this patent
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Abstract
A mannanase at least 75% identical to SEQ ID NO: 2, SEQ ID NO: 3, or SEQ ID NO: 4, a poly-nucleotide encoding mannanase, an expression construct comprising the polynucleotide, and a host cell comprising the polynucleotide or the expression construct.
Claims16
A mannanase variant comprising an amino acid sequence having at least 90% sequence identity to the full-length amino acid sequence of SEQ ID NO:
- 2, SEQ ID NO:
- 3, or SEQ ID NO:
- 4, wherein the mannanase variant comprises at least one amino acid substitution selected from the group consisting of D86N, Q89V/L, N96D, L101T/V, S103Y/E/A, K107N, N108G, N109Q/A, A112N, A119Y/H/T, N122S, A124E/C/D, S126E, S127A, N129M/L/F, S231Q/K/L/P/Y, 1233V, S235H/R/L/Q/N/Y, D244I/V/N, H254W, K255Y/H/R, E264Q/V, S270T, Q272I, K273T, N274E/C/Q, S281L, G286E/L/Q/A, W289F/M/H, S290A, N296H/F/Y, D301E/C/T, T309L, N312F/Y, A314P, L317T, and A319D/E, wherein the numbering is according to SEQ ID NO:
- 2, and wherein the mannanase variant has mannan-degrading activity.
1 more element
Dependencies are inferred from the claim text.