Chimeric filovirus vaccines
AssigneeKatholieke Universiteit Leuven
InventorsKai Dallmeier, Lorena Sanchez Felipe, Viktor Lemmens, Johan Neyts
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Abstract
The present invention relates to polynucleotides comprising a sequence of a live, infectious, attenuated Flavivirus wherein a nucleotide sequence encoding at least a part of a Filovirus glycoprotein is located at the intergenic region between the E and NS1 gene of said Flavivirus, such that a chimeric virus is expressed, characterised in that the encoded sequence C terminally of the E protein of said Flavivirus and N terminally of the signal peptide of the NS1 protein of said Flavivirus comprises in the following order: a further signal peptide of a Flavivirus NS1 protein, a filovirus glycoprotein wherein the N terminal signal peptide is absent, a TM domain of a flaviviral E protein.
Claims20
A polynucleotide comprising a sequence of a live, infectious, attenuated Flavivirus, the polynucleotide comprising:
- a nucleotide sequence that encodes at least a part of a Filovirus glycoprotein lacking an N terminal signal peptide,
- wherein:
- the nucleotide sequence is located at an intergenic region between an E gene of the Flavivirus and an NS1 gene of the Flavivirus, the E gene encoding an E protein, the NS1 gene encoding an NS1 protein, the NS1 protein comprising a signal peptide, such that a chimeric virus is expressed;
- and
- the nucleotide sequence is translatable such that the nucleotide sequence encodes a chimeric viral peptide, the chimeric viral peptide comprising:
- (a) a further signal peptide, positioned C terminally of the E protein, the further signal peptide having the same sequence as the signal peptide of the NS1 protein,
- (b) the Filovirus glycoprotein, positioned C terminally of the further signal peptide, and
- (c) a TM domain of a further Flaviviral E protein, positioned C terminally of the Filovirus glycoprotein;
- and
- the chimeric viral peptide is positioned N terminally of the NS1 protein of the Flavivirus.
A chimeric live, infectious, attenuated Flavivirus comprising:
- an E protein;
- an NS1 protein comprising a signal peptide;
- and
- a chimeric protein comprising:
- at least a part of a Filovirus glycoprotein lacking a functional signal peptide, wherein the Filovirus glycoprotein is inserted between the E protein and the NS1 protein;
- a further signal peptide, positioned C terminally of the E protein, wherein the further signal peptide has the same sequence as the signal peptide of the NS1 protein, and wherein the Filovirus glycoprotein is positioned C terminally of the further signal peptide;
- and
- a TM domain of a further Flaviviral E protein, positioned C terminally of the Filovirus glycoprotein,
- wherein the chimeric peptide is positioned N terminally of the NS1 protein.
Dependencies are inferred from the claim text.