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US 12,544,468 B2Granted
Method for decomposing peracetic acid and method for culturing microorganisms using same
AssigneeN-Cell Co., Ltd.
InventorsJong-Hee Kwon, Chang-Ho Cho
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Abstract
Provided is a method for decomposing peracetic acid and a method for culturing microorganisms using the decomposition method. The cultivation of microorganisms using the method of the present invention allows an effective removal of the peracetic acid used in a culture medium for sterilization.
Claims17
A method for culturing microorganisms, the method comprising:
- sterilizing a medium by adding peracetic acid to the medium containing a sugar and a nitrogen source to cause a reaction for sterilization;
- decomposing the peracetic acid by adding to the sterilized medium an iron ion, an alkali metal hydroxide, ethylenediaminetetraacetic acid (EDTA), and the sugar as accelerators for decomposition of the peracetic acid, causing a reaction between the peracetic acid and the accelerators to decompose the peracetic acid into acetic acid, water and oxygen;
- adding the sterilized medium to a bioreactor;
- and
- inoculating microorganisms into the sterilized medium and conducting a pure cultivation.
2 more elements
Dependencies are inferred from the claim text.