Composite high-brightness fluorophores with controllable spectra shapes and method of using composite highbrightness fluorophores
OwnerYoke Khin Yap, Dongyan Zhang +2inventors
InventorsYoke Khin Yap, Dongyan Zhang, Nazmiye Yapici, Xiuling Liu
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Abstract
A compound includes a first linker having a first end connected to the carrier, a second linker having a first end connected to the carrier, a third linker having a first end connected to the carrier, a first fluorescent entity connected to a second end of the first linker, a second fluorescent entity different from the first fluorescent entity connected to a second end of the second linker, and a biomolecule connected to a second end of the third linker. The biomolecule is configured to connect to a biomarker. A method of detecting biomarkers is also disclosed.
Claims20
A compound, comprising:
- a boron nitride carrier;
- a first linker having a first end connected to the carrier;
- a second linker having a first end connected to the carrier;
- a third linker having a first end connected to the carrier;
- a fourth linker having a first end connected to the carrier;
- a first fluorescent entity connected to a second end of the first linker;
- a second fluorescent entity different from the first fluorescent entity connected to a second end of the second linker;
- a third fluorescent entity different from the first and second fluorescent entities connected to a second end of the third linker, wherein a ratio of fluorescence intensities is x:y:z for the first, second, and third fluorescent entities, respectively, and further wherein at least two of x, y, and z is 1;
- and
- a biomolecule connected to a second end of the fourth linker, wherein the biomolecule is configured to connect to biomarker.
A method of detecting biomarkers, comprising:
- providing a plurality of fluorophores to a sample, each of the plurality of fluorophores including a biomolecule, a first fluorescent entity, a second fluorescent entity, and a third fluorescent entity linked to a carrier, wherein the biomolecule is configured to interact with a plurality of biomarkers in the sample, and whereby the fluorophore connects to the biomolecule;
- exciting the plurality of fluorophores in the sample with a laser;
- and
- detecting at least one of an identity and a quantity of the plurality of biomarkers in the sample based on a fluorescence spectra emitted by the excited plurality of fluorophores;
- wherein a ratio of fluorescence intensities is x:y:z for the first, second, and third fluorescent entities, respectively, and further wherein at least two of x, y, and z is 1.
Dependencies are inferred from the claim text.