Superparamagnetism-modified and neutrophil exosome biomimetic vesicle-based biological preparation for drug delivery, and preparation method thereof

A preparation method of a superparamagnetism-modified and neutrophil exosome biomimetic vesicle-based biological preparation for drug delivery, comprising the following steps:

• 1) conducting co-culture on peripheral blood neutrophils with an antitumor drug to obtain neutrophils for uptake of the drug;
• 2) conducting centrifugation on the neutrophils for uptake of the drug, and resuspending a bottom cushion to obtain a cell suspension;
• 3) conducting continuous physical extrusion and gradient filtration on the cell suspension to obtain a neutrophil exosome biomimetic vesicle-encapsulated drug suspension;
• wherein the gradient filtration is conducted with polycarbonate filter membranes of 1 μm, 400 nm, and 200 nm sequentially;
• 4) conducting centrifugation on the neutrophil exosome biomimetic vesicle-encapsulated drug suspension, resuspending a bottom neutrophil nano vesicle (NNV)-drug cushion to obtain an NNV-drug suspension;
• and
• 5) mixing the NNV-drug suspension with a superparamagnetism material for co-incubation and conducting magnetic separation on a co-incubated mixture to obtain the superparamagnetism-modified and neutrophil exosome biomimetic vesicle-based biological preparation for drug delivery.