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US 12,544,420 B2Granted
Electroporation-targeted delivery of ribosome inactivating protein gelonin into mammalian cells
AssigneeOld Dominion University Research Foundation
InventorsAndrei Pakhomov, Olga Pakhomova
Life of this patent
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Abstract
A method of treating a target tissue area with a ribosome-inactivating protein. The method comprises injecting the target tissue area with an effective dose of a ribosome-inactivating protein and administering electroporation therapy to the target tissue area.
Claims20
A method of ablating diverse malignant or benign tissues from a target tissue area with gelonin, the method comprising:
- injecting the target tissue area with an effective dose of gelonin to cause cell death within the target tissue area;
- and
- administering electroporation therapy to the target tissue area, the electroporation therapy including at least one pulse having a duration less than 1 ms.
A method of treating a target tissue area having malignant or benign tissues, the method comprising:
- injecting the target tissue area with an effective dose of a ribosome-inactivating protein;
- administering electroporation therapy to the target tissue area;
- and
- wherein the electroporation therapy comprises the administration of at least one pulse having a field strength of 9 kV/cm and a duration between 15 ns to 300 ns.
1 more element
A method of treating a subject having a cancerous tumor, the method comprising:
- injecting the cancerous tumor with a dose of a ribosome-inactivating protein to effectuate cell death within the cancerous tumor;
- administering electroporation therapy to the cancerous tumor via one or more electrodes powered by a pulse generator;
- and
- wherein the electroporation therapy comprises the administration of at least one pulse having a field strength between 0.40 kV/cm and 9 kV/cm and a duration between 15 ns and 1 ms-suitable to optimize a cytotoxic effect of the ribosome inactivating protein.
1 more element
Dependencies are inferred from the claim text.